We are pleased to inform you that the following article was published online in *Microbiology Resource Announcements on October 22, 2024:

Ueno, A., Sato, K., Tamamura, S., Murakami, T., Inomata, H., Tamazawa, S., Amano, Y., Miyakawa, K., Naganuma, T., & Igarashi, T. (2024).
Complete genome sequence of Thiomicrospira sp. strain V2501 isolated from 250 m below the ground level in Horonobe, Hokkaido, Japan*. Microbiology Resource Announcements, 13(10). [https://doi.org/10.1128/mra.00081-24](https://doi.org/10.1128/mra.00081-24)

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## Abstract

This paper presents the complete genome sequence of Thiomicrospira sp. strain V2501, a thiosulfate-oxidizing bacterium isolated from groundwater 250 meters below ground level in Horonobe, Hokkaido, Japan. The study provides key insights into the metabolic potential and phylogenetic position of this bacterium, contributing to our understanding of deep subsurface microbial ecosystems.

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## Key Themes

・Elucidation of microbial ecosystems in deep subsurface environments
・Metabolic characterization of Thiomicrospira sp. strain V2501 based on genomic data
・Discovery and taxonomic classification of novel microbial species

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## Key Findings

1. Isolation and Characterization of Thiomicrospira sp. V2501
Strain V2501 was isolated on December 15, 2009, from groundwater obtained via borehole 09-V250-M02, located 250 m below ground level at the Horonobe Underground Research Center. It is capable of chemolithoautotrophic growth, utilizing carbon dioxide and thiosulfate as substrates.

2. Genome Sequencing and Analysis
The complete genome of strain V2501 was obtained using the PacBio Sequel IIe system.
・Genome size : 2,240,851 bp
・GC content : 45.1 mol%
・Identified genes : 2,135 protein-coding genes, 9 RNA genes, and 43 tRNA genes

3. Phylogenetic Analysis
Phylogenomic analysis using TYGS revealed that strain V2501 is closely related to Thiomicrospira pelophila DSM 1534T, with an OrthoANIu value of 83.2%, which falls below the species-level cutoff of 95% ± 0.5%.


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Conclusion

The complete genome sequencing of Thiomicrospira sp. V2501 provides valuable insights into the metabolic mechanisms and ecological roles of subsurface microbes. The genomic data will facilitate further studies on the chemolithoautotrophic pathways of strain V2501 and its contributions to carbon and sulfur cycling in deep subsurface environments. Additionally, the phylogenetic analysis offers new perspectives on the diversity and evolutionary history of the Thiomicrospira genus.

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Data Availability
All relevant data are publicly available:
・16S rRNA gene sequence : Accession No. AB634592
・Sequence Read Archive (SRA) : DRA017671/DRR522933
・BioSample : SAMD00726318
・BioProject : PRJDB17264

These datasets have been deposited in NCBI/ENA/DDBJ for public access.